NAOVARAT, TARASUB.1, UMAISUNG, MUDA. 2,
KITTIWAT, SANGUANJEEN.2, THAWADCHAI, POJAN.2, WATCHARAPORN, DEVAKUL NA AYUTTHAYA.3,
TEERADECH, SURAMANA.3, THANES, FUANGFOO.4, THONGBAI, JANSEECHA.5
1Anatomy Unit, Department of Medical Sciences,
Faculty of Science, RSU.
2Pharmacy Program, Faculty of Pharmacy, RSU.
3Phramacology and Toxicology Unit, Department of Medical Sciences, Faculty of
Health Science and the Health Science Research Center, RSU.
4Division of Biopharmaceutical Sciences, Faculty of Pharmacy, RSU.
5Pathobiology Unit, Department of Medical Sciences, Faculty of Science, RSU.
(Cd) is an environmental pollutant whose toxic effects have been investigated
extensively. Acute exposure to Cd results primarily in liver damage. Myricetin,
quercetin, vitamin C and N-acetylcysteine (NAC) are effective antioxidants and
free radical scavengers. The aim of this work therefore was to investigate the
potential protective effects of these antioxidants against cadmium hepatotoxicity.
To assess the hepatotoxicity we determined the activities of aspartate aminotransferase
(AST) and alanine aminotransferase (ALT) in serum and histopathological changes
at the light microscopic level. Male Wistar rats received either cadmium chloride(CdCl2)
alone in the dose of 70, 140 and 210 mg/kg and pretreatment with quercetin (100
mg/kg), myricetin (10 mg/kg), vitamin C (100 mg/kg) and NAC (100 mg/kg) for
one hour before they were given CdCl2 at dose of 140 mg/kg by single
oral gavage. All rats were killed 24 hours after each treatment. The results
showed that activities of serum AST in CdCl2 140 and 210 mg/kg was
increased significantly (p < 0.01) and activities of serum
ALT in CdCl2 210 mg/kg was increased significantly (p <0.05)
as compared to control. The hydropic swelling of hepatocytes, vacuolation was
shown prominently in higher dose of CdCl2. These observations indicated
liver damages induced by CdCl2 occurred in dose-dependent manner.
Quercetin, vitamin C, NAC and myricetin showed no protective effects on Cdinduced
hepatotoxicity. A significant increase in activities of serum AST and ALT was
observed in treatment with myricetin as compared to CdCl2 treatment
(p <0.001). The cellular damages of hepatocytes was also found
in vitamin C, NAC and more prominence in myricetin treatment. In conclusion,
our study showed the hepatotoxicity induced by CdCl2 at 140 and
210 mg/kg and no protective effects by antioxidants used. In comparison, quercetin
had more effective than vitamin C, NAC and myricetin. Particularly, NAC and
myricetin had the prooxidant effect, it was not antioxidant in our experiment.
การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย ประจำปี 2548 Food & Chemical
Safety วันที่ 17-18 พฤศจิกายน 2548 โรงแรมแกรนด์ ทาวเวอร์อินน์ ถนนพระรามหก กรุงเทพฯ