Danai Tiwawech1, Adisak Sornprom2,
and Anant Karalak 3
1Research, 2Surgery and 3Pathology
Division, National Cancer Institute, Bangkok, Thailand.
Lung cancer (LCA) is a harmful disease that caused vastly losses in Thailand
yearly. The potential risk factor for screening of high-risk populations for
further early stage LCA detection followed by immediately treatment is an important
strategy to control this cancer. Polymorphism at codon 72 of the p53 tumor suppressor
gene (p53 ) has been reported to be associated with susceptibility
and prognosis of LCA. To focus on the genetic risk factor for LCA, we investigated
association between the p53 codon 72 polymorphism (Proline/Arginine,
Pro/Arg ) and LCA susceptibility in Thais.
Methods: DNA from peripheral
blood samples of 120 lung cancer patients and 120 age-matched healthy controls
was examined for genotype frequency of p53 codon 72 polymorphism
(Arg/Arg, Arg/Pro and Pro/Pro genotype) by using
polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
significant difference in the overall genotypes between cases and controls was
observed (P<0.001). The allele frequency of Pro in cases was significantly
higher than controls (P<0.001). Carriers with the Pro/Pro genotype
had a 6-fold increased risk for LCA when compared with those with Arg/Arg
genotype (P<0.001 with OR = 5.6, 95%CI = 2.3-13.9) but not with
Arg/Pro and Arg/Arg + Arg/Pro genotype.
Males and females with Pro/Pro genotype had similar risk for
LCA development (OR for males : females = 5.2 : 5.1). In addition, the Pro/Pro
genotype carriers had higher risk to develop a adenocarcinoma (ACA) than squamous
cell carcinoma of LCA (OR for ACA : SCC = 5.2 : 5.1). Moreover, carriers with
Pro/Pro genotype had higher risk to develop LCA stage IV than
stage III (OR for stage IV : stage III = 6.9 : 4.2).
Conclusions: The results
of our study suggest that the Pro/Pro genotype of p53
codon 72 polymorphism may associate with LCA susceptibility in Thai population.
The detection of p53 polymorphism may be a useful tool for screening
of high-risk group, early stage detection and prognosis of LCA.
การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย ประจำปี 2548 Food & Chemical
Safety วันที่ 17-18 พฤศจิกายน 2548 โรงแรมแกรนด์ ทาวเวอร์อินน์ ถนนพระรามหก กรุงเทพฯ