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ความรู้ทั่วไปเกี่ยวกับสิ่งเป็นพิษความรู้ทั่วไปที่นักวิชาการควรทราบพิษวิทยาคลีนิคก้าวทันโลกข้อมูลบริการแหล่งข้อมูลที่เกี่ยวข้อง
 
   
EFFECT OF PIPERINE ON CHROMOSOME STRUCTURE OF BONE MARROW CELLS IN MALE WISTAR RAT BY CYTOGENETIC METHOD.

WONGPA, SAREEYA.1, TEMCHAROEN, PUNYA.2, HIMAKOUN, LAKANA.2

1 Toxicology Graduate Programme, Faculty of Science, Mahidol University, Thailand
2 Department of Pathobiology, Faculty of Science, Mahidol University, Thailand


ABSTRACT

       Piperine is a major pungent substance and active component of black pepper (Piper nigrum  Linn.) and long pepper (Piper longum  Linn.). Both plants are used worldwide as household spices for instance, food additives and condiments. They are also used as important ingredients in folklore medicine in many Asian countries. Therefore, it is interested to study toxic effect of piperine particularly its genotoxic effect. In this study, the effect of piperine on the chromosome was investigated in rat bone marrow cells. Thirty male Wistar rats were randomly divided into 5 groups, 6 rats for each group. Group 1 (negative control), the animals were orally administered corn oil at a dose of 10 ml/kg BW. Group 2-4 (piperine treated groups), the animals were orally administered piperine at the doses of 100, 400 and 800 mg/kg BW, respectively. Group 5 (positive control), the animals were intraperitoneally injected with 50 mg/kg BW of cyclophosphamide. Twenty-four hours after treatment, the bone marrow cells were isolated from their femora for chromosomal analysis. The results of this study demonstrated that piperine at the given doses did not cause chromosome aberrations when compared to the control.

       In conclusion, piperine did not cause chromosome damage suggesting it had no genotoxic effect. The safety evaluation of piperine in human consumption will be discussed.

KEY WORDS: PIPERINE / GENOTOXIC EFFECT / CHROMOSOME ABERRATIONS / CYTOGENETIC METHOD

ที่มา :
การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย ประจำปี 2548 Food & Chemical Safety วันที่ 17-18 พฤศจิกายน 2548 โรงแรมแกรนด์ ทาวเวอร์อินน์ ถนนพระรามหก กรุงเทพฯ