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DNA damage detected by the comet assay in the white blood cells: Preliminary study.


Toxicology & Biochemistry Division, National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand

       The study was aimed at the detection in DNA level damage in white blood cells (WBC) of healthy adult. The alkaline single cell gel electrophoresis assay (known as the comet assay) in individual cells was adapted for detecting damaged DNA in WBC. The level of DNA damage was determined as the tail moment (TM) with comet analysis system. TM was assessed in cell before and after incubation in RPMI 1640 medium and CO2 at 37°C for 1 h to repair DNA breaks. Peripheral blood samples were donated from 5 females (aged 25-30 years), who were healthy, non-smokers, never had a deep x-ray, with no genetic disease and never worked in chemical/biohazard factory.

       Their averaged TM was 2.14 ± 0.3 pixels. There was a statistical significance different from positive control (61.4 ± 3.1 pixels) The spontaneous repair of DNA breaks showed a slight but significantly decrease in the TM (29.9 ± 3.9 pixels) of DNA damage WBC after incubation .This result will be the database in the future for comparing between TM of DNA damage in Medical Scientists who worked in office and chemical laboratory.

อาจารย์ รจนา ชุณหบัณฑิต สำนักงานวิจัยคณะแพทยศาสตร์โรงพยาบาลรามาธิบดี ถนนพระรามหก ราชเทวี กทม. 10400
E-mail: grrch@mahidol.ac.th

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การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย ประจำปี 2548 Food & Chemical Safety วันที่ 17-18 พฤศจิกายน 2548 โรงแรมแกรนด์ ทาวเวอร์อินน์ ถนนพระรามหก กรุงเทพฯ