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ความรู้ทั่วไปเกี่ยวกับสิ่งเป็นพิษความรู้ทั่วไปที่นักวิชาการควรทราบพิษวิทยาคลีนิคก้าวทันโลกข้อมูลบริการแหล่งข้อมูลที่เกี่ยวข้อง
 
   
ANTICLASTOGENIC ACTIVITIES OF THAI VEGETABLES USING MOUSE ERYTHROCYTE MICRONUCLEUS ASSAY

PIENGCHAI KUPRADINUN, ANONG TEPSUWAN, NUNTANA MEESIRIPUN AND WANNEE R. KUSAMRAN

Research Division, National Cancer Institute, Bangkok 10400, Thailand
E-mail :
pkupradi@health.moph.go.th

          We have previously reported that many kinds of vegetables commonly consumed in Thailand including Indian mulberry ( Morinda citrifolia  Linn.) leaves, Ivy gourd (Coccinia indica  Wright and Arm.) leaves and Sesbania (Sesbania grandiflora  Linn.) flowers contained antimutagens against indirect carcinogens/ mutagens such as aflatoxin B1(AFB1) and benz(a)pyrene [B(a)P]. The present study was aimed at the determination of anticlastogenic activity of the above vegetables against both direct and indirect-acting clastogens using in vivo mouse erythrocyte micronucleus assay. It was found that feeding of diets containing 12.5% and 25 % of Indian mulberry leaves in male mice 2 weeks prior to clastogen administration decreased the number of micronucleated peripheral reticulocytes (MNRETs) induced by both direct (mitomycin C, MMC) and indirect-acting clastogens (9,10-dimethyl-1,2-benzanthracene, DMBA). However, the effect was statistically significant only at high concentration. However, fruit juice of this vegetable at the concentration of 5 and 10 times of human use and fruit powder at the concentration of 5 times of human use caused a significant decrease in the number of MNRETs induced only by MMC. On the other hand, Ivy gourd leaves at 5% and 10% also significantly decreased MNRETs number induced by both DMBA and MMC. In addition, Sesbania flowers at 5% and 10% also caused a reduction in the number of MNRETs induced by both MMC and DMBA, however, the different was significant only at high concentration. The present study demonstrated that leaves of Indian mulberry and Ivy gourd as well as flowers of Sesbania possess anticlastogenic activity against both direct and indirect-acting carcinogens in the mouse. In addition, both juice and powder of Indian mulberry fruits could inhibit the clastogenicity of only direct-acting carcinogens. Interestingly, it can be concluded that all vegetables examined in this study may contain some anticlastogens.

ที่มา ABSTRACT นำเสนอที่ การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย ประจำปี 2547 อาหารกับความปลอดภัย วันศุกร์ที่ 17 ธันวาคม 2547 โรงแรมมิราเคิลแกรนด์ กรุงเทพฯ