1, CHANIPHUN BUTRYEE 1, PIENGCHAI KUPRADINUN 2
1 Institute of
Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
2 Research Division, National Cancer Institute, Bangkok 10400, Thailand
Background: Amaranthus viridis
and Amaranthus tricolor are in family Amaranthaceae. A.viridis
is widely grown and consumed in Northeastern and Southern parts of Thailand.
It is served as boiled or blanced and consumed together with Thai chili paste
or cooked as soup. A.tricolor is widely consumed in all parts of
Thailand and it also served in international cuisine.
Objectives: The clastogenic and anticlastogenic
potentials of raw and cooked Amaranthus viridis.(Pak Khom Had)
and A.tricolor ( Pak Khom Suan) induced by mitomycin C (MMC), a direct
acting carcinogen, and 9,10-dimethyl-1,2-benzanthracene (DMBA), an indirect
acting carcinogen, were determined using in vivo erythrocyte micronucleus
Methods: Three samples of Amaranthus ,
one raw, one blanched and the other boiled, were extracted with methanol. Each
extract (100 and 200 mg/kg) for each sample was then administered to male mice
by intragastric intubation for 2 weeks prior to the administration of MMC intraperitoneally
and DMBA intragastrically. The carcinogens were introduced to the mice then
blood was collected from all groups at 0, 24, 48 and 72 hours and was analyzed
for the number of micronucleated peripheral reticulocytes (MNRETs). A control
group received the carcinogens only.
Results: The ingestion of 100 and 200 mg/kg
vegetables extracts, both raw and cooked (blanched and boiled) A. viridis
and A. tricolor , for 2 weeks had no significant effect on the micronucleus
formation in erythrocyte micronucleuse assay. For the anticlastogenicity test,
all of them caused a significant reduction in the number of MNRETs induced by
MMC and DMBA when compared with the control group. Both A. viridis and A. tricolor
extracts had a similar effect on MMC and DMBA induced MNRETs. The extracts of
raw vegetable showed the highest anticlastogenic potential against MMC and DMBA.
However, the inhibition of MMC induced MNRETs was markedly reduced in the blanched
vegetable and less in case of boiled vegetable. In case of DMBA induced MNRETs,
the inhibition by blanched and boiled vegetables was not different.
Conclusion: A.viridis and A.tricolor
had no clastogenicity. Interestingly, however they showed significant anticlastogenic
activity against both direct and indirect-acting carcinogens.
Key words: Clastogenicity-Anticlastogenicity-Amaranth-Mitomycin
DMBA-Erythrocyte Micronucleus Assay
ที่มา: ABSTRACT นำเสนอที่ การประชุมวิชาการสมาคมพิษวิทยาแห่งประเทศไทย
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